Boes A, Olatunji S, Breukink E, Terrak M Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN. MBio. 2019 Jan 8;10(1). pii: e01912-18. doi: 10.1128/mBio.01912-18

van Willigen M, Vonk AM, Yeoh HY, Kruisselbrink E, Kleizen B, van der Ent CK, Egmond MR, de Jonge HR, Braakman I, Beekman JM, van der Sluijs P Folding-function relationship of the most common cystic fibrosis-causing CFTR conductance mutants. Life Sci Alliance. 2019 Jan 18;2(1). pii: e201800172. doi: 10.26508/lsa.201800172. Print 2019 Feb.

Anaokar S, Kodali R, Jonik B, Renne MF, Brouwers JFHM, Lager I, de Kroon AIPM, Patton-Vogt J.
The glycerophosphocholine acyltransferase Gpc1 is part of a phosphatidylcholine (PC)-remodeling pathway that alters PC species in yeast.
J Biol Chem. 2018 Dec 4. pii: jbc.RA118.005232. doi: 10.1074/jbc.RA118.005232. [Epub ahead of print]

Dominguez Pardo JJ, Koorengevel MC, Uwugiaren N, Weijers J, Kopf AH, Jahn H, van Walree CA, van Steenbergen MJ, Killian JA
Membrane Solubilization by Styrene-Maleic Acid Copolymers: Delineating the Role of Polymer Length. Biophys J. 2018 Jul3; 115(1):129-138 doi 10.1016/bpj 2018.05.032

Egan AJF, Maya-Martinez R, Ayala I, Bougault CM, Banzhaf M, Breukink E, Vollmer W, Simorre JP
Induced conformational changes activate the peptidoglycan synthase PBP1B. Mol. Microbiol. 2018; 110:335. doi: 10.1111/mmi.14082

Henrich E, Löhr F, Pawlik G, Peetz O, Dötsch V, Morgner N, de Kroon AIPM, Bernhard F
Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism. Biochemistry 2018; 57:40. doi: 10.1021/acs.biochem.8b00807

Hernández-Rocamora VM, Otten CF, Radkov A, Simorre JP, Breukink E, VanNieuwenhze M, Vollmer W.
Coupling of polymerase and carrier lipid phosphatase prevents product inhibition in peptidoglycan synthesis. Cell Surf. 2018; 2:1. doi: 10.1016/j.tcsw.2018.04.002

Hoffmann ARF, Saravanan MS, Lequin O, Killian JA, Khemtemourian L
A single mutation on the human amyloid polypeptide modulates fibril growth and affects the mechanism of amyloid-imduced membrane damage. Biochim Biophys Acta 2018 Feb 27 doi 10.1016/j.bbamem.2018.02.018 (Epub ahead of print]

Jiang H, Tang X, Zhou Q, Zou J, Li P, Breukink E, Gu Q
Plantaricin NCB from Lactobacillus plantarum causes cell membrane disruption to Micrococcus luteus without targeting lipid II. Apl. Microbiol Biotechnol. 2018 Jul 7 doi: 10.1007.s00253-018-9182-3 (Epub ahead of print]

Kopf AH, Koorengevel MC, van Walree CA, Dafforn TR, Killian JA
A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers. Chem.Phys.Lipids 2018 Dec 1;218:85-90. doi: 10.1016/j.chemphyslip.2018.11.011. [Epub ahead of print]

Liu Y, Moura ECCM, Dörr JM, Scheidelaar S, Heger M, Egmond MR, Killian JA, Mohammadi T, Breukink E.
Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers.PLoS One. 2018 Nov 5;13(11):e0206692. doi: 10.1371/journal.pone.0206692. eCollection 2018

Medeiros-Silva J, Jekhmane S, Lucini Paioni A, Gawarecka K, Baldus M, Swiezewska E, Breukink E, Weingarth M
High-resolution NMR studies of antibiotics in cellular membranes Nature Communications 2018; 9:3963. doi: 10.1038/s41467-018-06314-x

Montón Silva A, Otten C, Biboy J, Breukink E, VanNieuwenhze M, Vollmer W, den Blaauwen T.
The Fluorescent D-Amino Acid NADA as a Tool to Study the Conditional Activity of Transpeptidases in Escherichia coli.Front Microbiol. 2018 Sep 4;9:2101. doi: 10.3389/fmicb.2018.02101. eCollection 2018.

Morlot C, Straume D, Peters K, Hegnar OA, Simon N, Villard AM, Contreras-Martel C, Leisico F, Breukink E, Gravier-Pelletier C, Le Corre L, Vollmer W, Pietrancosta N, Håvarstein LS, Zapun A.
Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae.Nat Commun. 2018 Aug 9;9(1):3180. doi: 10.1038/s41467-018-05602-w.

Pazos M, Peters K, Casanova M, Palacios P, VanNieuwenhze M, Breukink E, Vicente M, Vollmer W
Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nat Commun. 2018 Nov 30;9(1):5090. doi: 10.1038/s41467-018-07559-2.

Renne MF, de Kroon AIPM
The role of phospholipid molecular species in determining the physical properties of yeast membranes. FEBS Letters 2018; 592:13300-1345. doi: 10.1002/1873-3468.12944. PMID: 5947837

Bersch B, Dörr JM, Hessel A, Killian JA, Schanda P
Proton-Detected Solid-State NMR Spectroscopy of a Zinc Diffusion Facilitator Protein in Native Nanodiscs. Angew Chem Int Ed Engl. 2017 Jan 27. doi: 10.1002/anie.201610441. PMID: 28128538

Broekgaarden M, Weijer R, van Wijk AC, Cox RC, Egmond MR, Hoebe R, van Gulik TM, Heger M
Photodynamic Therapy with Liposomal Zinc Phthalocyanine and Tirapazamine Increases Tumor Cell Death via DNA Damage. J Biomed Nanotechnol. 2017 Feb;13(2):204-20. PMID: 29377650

Calvez P, Breukink E, Roper DI, Dib M, Contreras-Martel C, Zapun A
Substitutions in PBP2b from β-lactam resistant Streptococcus pneumoniae have different effects on enzymatic activity and drug reactivity. J Biol Chem. 2017 Jan 6. pii: jbc.M116.764696. doi: 10.1074/jbc.M116.764696. PMID: 28062575

De Kroon AIPM
Lipidomics in research on yeast membrane lipid homeostasis. Biochim Biophys Acta. 2017 Aug;1862(8):797-799. doi: 10.1016/j.bbalip.2017.02.007. Epub 2017 Feb 17. PMID: 28219720

Dominguez Pardo JJ, Dörr JM, Iyer A, Cox RC, Scheidelaar S, Koorengevel MC, Subramaniam V, Killian JA
Solubilization of lipids and lipid phases by the styrene-maleic acid copolymer. Eur Biophys J. 2017 Jan;46(1):91-101. doi: 10.1007/s00249-016-1181-7. PMID: 27815573

Dominguez Pardo JJ, Dörr JM, Renne MF, Ould-Braham T, Koorengevel MC, van Steenbergen MJ, Killian JA
Thermotropic properties of phosphatidylcholine nanodiscs bounded by styrene-maleic acid copolymers. J. Chem Phys Lip 2017 Nov;208:58-64. doi: 10.1016/j.chemphyslop.2017.08.0101

Dörr JM, van Coevorden MH, Hoogenraad CC, Killian JA
Solubilization of human cells by the styrene-maleic acid copolymer: insights from fluorescence microscopy. Biochim Biophys Acta 2017 Nov;1859(11):2155-2160. doi: 10.1016/j.bbamem.2017.08.010.

't Hart P, Wood T, Hajmohammadebrahimtehrani K, van Harten R, Sleszynska M, Breukink E, Martin N
De novo identification of lipid II binding lipopeptides with antibacterial activity against vancomycin-resistant bacteria. Chem. Sci. 2017;8:7991-7997 doi: 10.1039/c7sc03413.

Khemtemourian L, Guillemain G, Foufelle F, Killian JA
Residue specific effects of human islet polypeptide amyloid on self-assembly and on cell toxicity. Biochimie. 2017 Aug; pii: S0300-9084(17)30186-4. doi: 10.1016/j.biochi.2017.07.015. PMID: 28778718

Leclercq S, Derouaux A, Olatunji S, Fraipont C, Egan AJ, Vollmer W, Breukink E, Terrak M
Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis. Sci Rep. 2017 Feb 24;7:43306. doi: 10.1038/srep43306. PMID: 28233869

Parmar A, Prior SH, Iyer A, Vincent CS, Van Lysebetten D, Breukink E, Madder A, Taylor EJ, Singh I
Defining the molecular structure of teixobactin analogues and understanding their role in antibacterial activities. Chem Commun (Camb). 2017 Jan 26. doi: 10.1039/c6cc09490b. PMID: 28124045

Reiniers MJ, van Golen RF, Bonnet S, Broekgaarden M, van Gulik TM, Egmond MR, Heger M
Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays. Anal Chem. 2017 Apr 4;89(7):3853-3857. doi: 10.1021/acs.analchem.7b00043. Epub 2017 Mar 15. PMID: 28224799

Swainsbury DJK, Scheidelaar S, Foster N, van Grondelle R, Killian JA, Jones MR
The effectiveness of styrene-maleic acid (SMA) copolymers for solubilisation of integral membrane proteins from SMA-accessible and SMA-resistant membranes. Biochim Biophys Acta. 2017 Jul 24. pii: S0005-2736(17)30236-5. doi: 10.1016/j.bbamem.2017.07.011. PMID: 28751090. Epub 2017 Jul 24

Broekgaarden M, van Vught R, Oliveira S, Roovers RC, van Bergen En Henegouwen PM, Pieters RJ, Van Gulik TM, Breukink E, Heger M
Site-specific conjugation of single domain antibodies to liposomes enhances photosensitizer uptake and photodynamic therapy efficacy. Nanoscale 2016 8(12):6490-4

Cleverley RM, Rismondo J, Lockhart-Cairns MP, Van Bentum PT, Egan AJ, Vollmer W, Halbedel S, Baldock C, Breukink E, Lewis RJ
Subunit Arrangement in GpsB, a Regulator of Cell Wall Biosynthesis Microb.Drug Resist. 2016 Jun 3 [Epub ahead of print]

Dörr JM, Scheidelaar S, Koorengevel MC, Dominguez JJ, Schäfer M, van Walree CA, Killian JA
The styrene-maleic acid copolymer: a versatile tool in membrane research Eur Biophys J. 2016 Jan;45(1):3-21.

Gruber JM, Scheidelaar S, van Roon H, Dekker JP, Killian JA, van Grondelle R
Photophysics in single light-harvesting complexes II: from micelle to native nanodisks Proc. of SPIE 2016 Vol. 9714 97140A-1

Kleijn LH, Oppedijk SF, 't Hart P, van Harten RM, Martin-Visscher LA, Kemmink J, Breukink E, Martin NI
Total Synthesis of Laspartomycin C and Characterization of Its Antibacterial Mechanism of Action J.Med.Chem. 2016 59(7):3569-74

Liu Y, Rodrigues JP, Bonvin AM, Zaal EA, Berkers CR, Heger M, Gawarecka K, Swiezewska E, Breukink E, Egmond MR
New insight in the catalytic mechanism of bacterial MraY from enzyme kinetics and docking studies J.Biol.Chem. 2016 291(29):15057-68

Oppedijk SF, Martin NI, Breukink E
Hit 'em where it hurts: The growing and structurally diverse family of peptides that target lipid-II Biochim Biophys Acta. 2016 1858(5): 947-57

Renne MF, Bao X, De Smet CH, de Kroon AI
Lipid Acyl Chain Remodeling in Yeast Lipid Insights. 2016 Jan 19;8(Suppl 1):33-40. doi: 10.4137/LPI.S31780. eCollection 2015. Review

Scheidelaar S, Koorengevel MC, van Waalree CA, Dominguez JJ, Dörr JM, Killian JA
Effect of Polymer Composition and pH on Membrane Solubilization by Styrene-Maleic Acid Copolymers. Biphysical Journal 2016 Nov 1; 111(9): 1974-1986

't Hart P, Oppedijk S.F., Breukink E, Martin N.I.
New Insights into Nisin's Antibacterial Mechanism Revealed by Binding Studies with Synthetic Lipid II Analogues Biochemistry 2016 55(1): 232-7

van 't Veer IL, Leloup NOL, Egan AJF, Janssen, BJC, Martin NI, Vollmer W, Breukink E
Site specific immobilization of the peptidoglycan synthase PBP1B on a surface plasmon resonance chip surface. Chembiochem 2016 Oct 6 [Epub ahead of print]

Blanchaert B, Wyseure T, Breukink E, Adams E, Declerck P, Van Schepdael A
Development of a liquid chromatography/mass spectrometry assay for the bacterial transglycosylation reaction through measurement of Lipid II Electrophoresis. 2015 Sep 3 [Epub Ahead of of Print]

Bury D, Dahmane I, Derouaux A, Dumbre S, Herdewijn P, Matagne A, Breukink E, Mueller-Seitz E, Petz M, Terrak M
Positive cooperativity between acceptor and donor sites of the peptidoglycan glycosyltransferase. Biochem Pharmacol. 2015 Jan 15;93(2):141-50

Cybulski LE, Ballering J, Moussatova A, Inda ME, Vazquez DB, Wassenaar TA, de Mendoza D, Tieleman DP, Killian JA
Activation of the bacterial thermosensor DesK involves a serine zipper dimerization motif that is modulated by bilayer thickness.Proc Natl Acad Sci U S A. 2015 ; 112(20):6353-8

Egan AJ, Biboy J, Van 't Veer I, Breukink E, Vollmer W
Activities and regulation of peptidoglycan synthases. Philos Trans R Society Lond B Biology Science. 2015 Oct 5; 370(1679)

Furse S, de Kroon AI
Phosphatidylcholine's functions beyond that of a membrane brick Mol Membr Biol. 2015 32(4):117-9

Furse S, Egmond MR, Killian JA
Isolation of lipids from biological samples. Mol Membr Biol. 2015 32(3): 55-64

Furse S, Wienk H, Boelens R, de Kroon AI, Killian JA
E. coli MG1655 modulates its phospholipid composition through the cell cycle FEBS let 2015 Sep 14;589(19 PT B):2726-30

Gray AN, Egan AJ, Van't Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar AF, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W
Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division. Elife 2015 May7;4

Koopmans T, Wood TM, 't Hart P, Kleijn LH, Hendrickx AP, Willems RJ, Breukink E, Martin NI
Semisynthetic Lipopeptides Derived from Nisin Display Antibacterial Activity and Lipid II Binding on Par with That of the Parent Compound. J Am Chem Soc. 2015 Jul 29;137(29):9382-9. doi: 10.1021/jacs.5b04501.

Olthof ED, Gulich AF, Renne MF, Landman S, Joosten LA, Roelofs HM, Wanten GJ
Immune activation by medium-chain triglyceride-containing lipid emulsions is not modulated by n-3 lipids or toll-like receptor 4. Toxicol In Vitro 2015 Oct;29(7): 1851-8

Scheidelaar S, Koorengevel MC, Dominguez Pardo JD, Meeldijk JD, Breukink E, Killian JA
Molecular model for the solubilization of membranes into nanodisks by styrene maleic Acid copolymers. Biophys J. 2015 Jan 20;108(2):279-90

Slootweg JC, van Herwerden EF, van Doremalen MF, Breukink E, Liskamp RM, Rijkers DT
Synthesis of nisin AB dicarba analogs using ring-closing metathesis: influence of sp(3)versus sp(2) hybridization of the a-carbon atom of residues dehydrobutyrine-2 and dehydroalanine-5 on the lipid II binding affinity. Org Biomol Chem. 2015 May 19;13(21):5997-6009

Sosic I, Anderluh M, Sova M, Gobec M, Mlinaric Rašcan I, Derouaux A, Ana Amoroso A, Terrak M, Breukink E, and Gobec S
Structure-Activity Relationships of Novel Tryptamine-Based Inhibitors of Bacterial Transglycosylase. J Med Chem. 2015 Dec24;58(24):9712-21

Spelbrink RE, Lensing H, Egmond MR, Giuseppin ML
Potato Patatin Generates Short-Chain Fatty Acids from Milk Fat that Contribute to Flavour Development in Cheese Ripening Appl Biochem Biotechnol. 2015 Mar 27.


Membrane Biochemistry & Biophysics is part of the Department of Chemistry of Utrecht University, The Netherlands. It is part of both the Institute of Biomembranes and the Bijvoet Center

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